注册 登录  
 加关注
   显示下一条  |  关闭
温馨提示!由于新浪微博认证机制调整,您的新浪微博帐号绑定已过期,请重新绑定!立即重新绑定新浪微博》  |  关闭

wangyufeng的博客

祝愿BB 健康开心快乐每一天

 
 
 

日志

 
 

Control of cotton fibre elongation by a homeodomain transcription factor GhHOX3  

2014-12-16 19:25:09|  分类: 文献学习 |  标签: |举报 |字号 订阅

  下载LOFTER 我的照片书  |
来自中科院上海生命科学研究院、南京农业大学、爱荷华州立大学等机构的研究人员证实,一种叫做GhHOX3的同源异型转录因子控制了棉花纤维伸长。这一研究发现发表在11月自然通讯》(Nature Communications)杂志上。

棉纤维由胚珠外珠被单个表皮细胞分化发育而成。纤维细胞起始分化后,一般不再分裂,而是持续地伸长,最终长度可达其直 径的1000-3000倍。纤维发育历经起始分化、纤维伸长及初生壁合成、次生壁合成及脱水成熟4个时期。尽管研究人员在对控制棉纤维起始分化的调控因子 研究中取得了一些进展,揭示出许多的因子影响了纤维细胞生长,迄今为止有关纤维伸长的关键调控因子及调控机制仍有待阐明。

在这篇新文章中研究人员报告称,发现一种同源异型-亮氨酸拉链 (homeodomain-leucine zipper protein, HD-Zip)转录因子GhHOX3控制了棉纤维伸长。GhHOX3基因定位在异源四倍体棉花品种的A12和D12同源染色体上,与纤维长度数量性状基因 座(QTLs)相关联。沉默GhHOX3可大大减短纤维长度(>80%),而过表达GhHOX3则可导致纤维长度伸长。

Abstract:

Cotton fibres are unusually long, single-celled epidermal seed trichomes and a model for plant cell growth, but little is known about the regulation of fibre cell elongation. Here we report that a homeodomain-leucine zipper (HD-ZIP) transcription factor, GhHOX3, controls cotton fibre elongation. GhHOX3 genes are localized to the 12th homoeologous chromosome set of allotetraploid cotton cultivars, associated with quantitative trait loci (QTLs) for fibre length. Silencing of GhHOX3 greatly reduces (>80%) fibre length, whereas its overexpression leads to longer fibre. Combined transcriptomic and biochemical analyses identify target genes of GhHOX3 that also contain the L1-box cis-element, including two cell wall loosening protein genes ?GhRDL1 and ?GhEXPA1. GhHOX3 interacts with ?GhHD1, another homeodomain protein, resulting in enhanced transcriptional activity, and with cotton DELLA, ?GhSLR1, repressor of the growth hormone gibberellin (GA). ?GhSLR1 interferes with the GhHOX3–?GhHD1 interaction and represses target gene transcription. Our results uncover a novel mechanism whereby a homeodomain protein transduces GA signal to promote fibre cell elongation.

Control of cotton fibre elongation by a homeodomain transcription factor GhHOX3 - 喜欢吃桃子 - wangyufeng的博客
 (ae) Phenotypic analysis of 35S::GhHOX3 transgenic plants. Wild-type (WT) cotton and three transgenic lines are shown, of which two (5–8 and 2–3) exhibited co-suppression. (a) Images of fibre of 35S::GhHOX3 lines, T2 generation. Scale bar, 1?cm. (b) Scanning electron microscope (SEM) images of the ovule (0–2 DPA) of the co-suppression line 5–8 and the WT. Scale bars, 50?μm. (c) Mean fibre length at 0–4 DPA (mean±s.e.m., n>100). (d) Fibre of homozygous and heterozygous35S::GhHOX3 co-suppression line 5–8 (T6 generation). Heterozygotes were generated by back-crossing the T5 plants to the WT. Scale bar, 1?cm. (e) Northern blot of GhHOX3 expression in fibre (9 DPA) of the WT and the 35S::GhHOX3 (5–8 and 1–6) lines. (f) Images of fibre from a T2 plant of the 35S::GhHOX3g (genomic) transgenic line. Scale bar, 1?cm. (g) Mature fibre length of a 35S::GhHOX3g cotton line (mean±s.e.m., n=30, ***P<0.001, Student’s t-test). (h) Quantitative reverse transcription-PCR analysis of GhHOX3 expression levels in 35S::GhHOX3g ovule (O) and fibre (F), T2; DPA or D, DPA. Data are shown as mean±s.e.m. (n=3).
Full text:
http://www.nature.com/ncomms/2014/141121/ncomms6519/full/ncomms6519.html

 

  评论这张
 
阅读(513)| 评论(0)
推荐 转载

历史上的今天

在LOFTER的更多文章

评论

<#--最新日志,群博日志--> <#--推荐日志--> <#--引用记录--> <#--博主推荐--> <#--随机阅读--> <#--首页推荐--> <#--历史上的今天--> <#--被推荐日志--> <#--上一篇,下一篇--> <#-- 热度 --> <#-- 网易新闻广告 --> <#--右边模块结构--> <#--评论模块结构--> <#--引用模块结构--> <#--博主发起的投票-->
 
 
 
 
 
 
 
 
 
 
 
 
 
 

页脚

网易公司版权所有 ©1997-2017