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RNAi and DsiRNA: Pathway, Mechanism, and Design  

2013-05-03 09:05:16|  分类: DNA实验基础 |  标签: |举报 |字号 订阅

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RNA interference (RNAi) is a conserved pathway found in most eukaryotes where dsRNAs suppress expression of genes with complementary sequences [1, 2]. RNAi has become the experimental tool of choice for studying the effects of gene silencing.

Definitions

dsRNA—double-stranded RNA

Dicer—an endoribonuclease that degrades long dsRNAs into small, effector molecules called siRNAs

siRNA—small interfering RNA

RISC—RNA-Induced Silencing Complex

Passenger strand—the sense strand of the siRNA; will be degraded

Guide strand—the antisense strand of the siRNA; will be incorporated into RISC

DsiRNAs—Dicer-substrate RNAs; chemically synthesized 27mer duplex RNAs that have increased potency in RNA interference compared to traditional 21mer siRNAs.

RNAi and DsiRNA: Pathway, Mechanism, and Design - 喜欢吃桃子 - wangyufeng的博客
 Pathways Involved in Degradative RNAi. Double-stranded RNAs (dsRNAs) are cleaved by Dicer into small interfering RNAs (siRNAs) that are 21–23 bp duplexes with 3’ overhangs and 5’-phosphates. The siRNAs then enter the RNA-induced silencing complex (RISC) where the guide strand directs cleavage of the target RNA

Tools to Understand Gene Function

RNAi is a powerful tool for studying gene silencing and its effects. Here we have described critical design parameters, the importance of controlling for off-target effects, and considerations for in vivo gene silencing. Advancements to the technology, such as IDT DsiRNAs, have led to even greater advancements in the potency of RNA interference. Such tools will continue to provide the means to study the role specific genes play through the effects of silencing them. For more information on DsiRNAs from IDT, visit the Custom DsiRNA product page.

Fulltext:http://www.idtdna.com/pages/decoded/decoded-articles/core-concepts/decoded/2011/09/12/rnai?utm_source=Google%2B&utm_medium=Update&utm_campaign=decoded%2B1.3


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