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水稻直立型密穗基因DEP3的图位克隆:Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice(Oryza sativa L.)  

2012-03-09 18:41:14|  分类: 数量遗传学 |  标签: |举报 |字号 订阅

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     穗型是一个重要的农艺性状,主要由初级和次级枝梗的形态、数量和长度决定。dep3 是一种直立型密穗粳稻突变体,其穗从开花到完熟都保持直立状态,而野生型稻穗在花后就开始下垂,此外,突变体的穗长、粒形以及每穗粒数等性状也发生改变。与野生型相比,dep3 突变体的维管束更小、茎更粗,这解释了穗直立的表型。直立型密穗表型是由单隐性基因dep3 控制。通过图位克隆技术鉴定出候选基因DEP3,预测其编码一个含有patatin样磷脂酶A2PLA2)超家族结构域的蛋白。突变体等位基因在LOC_Os06g46350处缺失408bp,包括第3外显子编码区的后47bp以及3'端的非翻译区的前361bp。综上所述,PLA2可能在维管束形成中发挥重要作用。

水稻直立型密穗基因DEP3的图位克隆:Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice(Oryza sativa L.) - 喜欢吃桃子 - wangyufeng的博客
 

Phenotype of the dep3 mutant.

 
       Architecture of the rice inflorescence, which is determined mainly by the morphology, number and length of primary and secondary inflorescence branches, is an important agronomical trait. In the current study, we characterized a novel dense and erect panicle (EP) mutant, dep3, derived from the Oryza sativa ssp. japonica cultivar Hwacheong treated with N-methyl-N-nitrosourea. The panicle of the dep3 mutant remained erect from flowering to full maturation, whereas the panicle of the wild type plant began to droop after flowering. The dep3 mutation also regulated other panicle characteristics, including panicle length, grain shape and grain number per panicle. Anatomical observations revealed that the dep3 mutant had more small vascular bundles and a thicker culm than wild type plants, explaining the EP phenotype. Genetic analysis indicated that the phenotype with the dense and EP was controlled by a single recessive gene, termed dep3. The DEP3 gene was identified as the candidate via a map-based cloning approach and was predicted to encode a patatin-like phospholipase A2 (PLA2) superfamily domain-containing protein. The mutant allele gene carried a 408 bp genomic deletion within LOC_Os06g46350, which included the last 47 bp coding region of the third exon and the first 361 bp of the 3′-untranslated region. Taken together, our results indicated that the patatin-like PLA2 might play a significant role in the formation of vascular bundles, and that the dep3 mutant may provide another EP resource for rice breeding programs.
Yongli Qiao;Rihua Piao;Jinxia Shi;Song-I Lee;Wenzhu Jiang;Baek-Ki Kim;Joohyun Lee;Longzhi Han;Wenbo Ma;Hee-Jong Koh
  Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice (Oryza sativa L.) Theoretical and Applied Genetics, 2011, 122(7): 1439-1449

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