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使用电子蒸腾计研究植物实时水分利用率:AN ELECTRONIC POTOMETER FOR STUDYING PLANT WATER USE IN REAL TIME  

2011-09-29 00:34:05|  分类: 植物耐铝机理 |  标签: |举报 |字号 订阅

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In order to achieve accurate real-time monitoring of plant water use by means of a simple, multi-channel apparatus, we developed a Micro-Electronic Potometer (MEP). The instrument is built from six units each comprising two parallel vessels joined by a tube; one vessel accommodates a hydroponically grown plant; the other contains a float connected to a high-accuracy linear variable differential transducer (LVDT). Very small changes in water level are recorded by the LVDT output. The potometer responds to a change in water use as little as 1-mL. The instrument was tested by monitoring the water uptake rates of 3-week-old tobacco seedlings and detached tobacco plant organs, following exposure to changes in light intensity and addition of NaCl, HgCl2 or abscisic acid to the root medium. All of these treatments elicited the expected responses in water use within 3-20 min. The advantage of the instrument was also demonstrated in distinguishing between water use of several plant species, including Arabidopsis thaliana cv. Colombia C24 and young pine trees, under normal and stress conditions. This potometer enables the immediate, real-time evaluation of continuous changes in plant water use in response to modifications in the environments of the roots or shoots. Thus, it is a useful, low-cost laboratory instrument for diverse physiological research into plant-water relations.

by Refael Aharon, Gad Galili, Abraham Blum and Yoram Kapulnik

 Department of Plant Science, the Weizmann Institute of Science, Rehovot 76100, Israel;

and 1Agronomy and Natural Resources Dept., ARO, the Volcani Center, Bet Dagan 50250, Israel;

 2presently at www.plantstress.com

3Corresponding author email: kapulnik@volcani.agri.gov.il

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