注册 登录  
 加关注
   显示下一条  |  关闭
温馨提示!由于新浪微博认证机制调整,您的新浪微博帐号绑定已过期,请重新绑定!立即重新绑定新浪微博》  |  关闭

wangyufeng的博客

祝愿BB 健康开心快乐每一天

 
 
 

日志

 
 

ClustalW:A multiple sequence alignment program for DNA or proteins  

2011-11-05 13:08:16|  分类: 生物信息分析 |  标签: |举报 |字号 订阅

  下载LOFTER 我的照片书  |

Software at http://www.ebi.ac.uk/Tools/clustalw2/

 

Step 1: Prepare your nucleotide or protein sequences.

The program accepts sequences in the following formats :
NBRF/PIR, EMBL/UniProt, Pearson (FASTA), GDE, ALN/ClustalW, GCG/MSF, RSF.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

Step 2: Upload your file.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

Step 3: Run this program.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客 


Step 4: Progressing…

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

 

 

 

 

 

 

 

 

 

Step 5: Show the alignment result.

The alignment will appear on the results page along with details of scores and guide trees.

The alignment can be obtained on its own by clicking on the alignment file option at the top (.aln).

This file can be opened in a separate window or saved to a file.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

Step 6: Get the color version of the alignment.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

 

 

 

 

 

 

 

 

 

 

 

 


Step 7: Start Jalview.

Jalview is a mulitple alignment editor that provided as an option when you retrieve a multiple alignment from ClustalW2.

To use it, just click on the Jalview gif. It allows you to do things like:

  • Use many different colour schemes
  • Read and write alignments in a variety of formats
  • Order the alignment according to different criteria
  • Draw UPGMA and NJ trees depending on percent identity criteria
  • Remove gapped columns
  • Perform Smith-Waterman alignment of selected sequences
  • Insert/delete gaps using the mouse
  • Cluster sequences using principle component analysis (PCA)

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

Step 8: View scores table.

A pairwise score is calculated for every pair of sequences that are to be aligned.

These scores are presented in a table in the results.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客

 

 

 

 

 

 

 

 

 

 

 

 


Step 9: View guide tree.

ClustalW:A multiple sequence alignment program for DNA or proteins - 喜欢吃桃子 - wangyufeng的博客
  评论这张
 
阅读(1764)| 评论(0)
推荐 转载

历史上的今天

在LOFTER的更多文章

评论

<#--最新日志,群博日志--> <#--推荐日志--> <#--引用记录--> <#--博主推荐--> <#--随机阅读--> <#--首页推荐--> <#--历史上的今天--> <#--被推荐日志--> <#--上一篇,下一篇--> <#-- 热度 --> <#-- 网易新闻广告 --> <#--右边模块结构--> <#--评论模块结构--> <#--引用模块结构--> <#--博主发起的投票-->
 
 
 
 
 
 
 
 
 
 
 
 
 
 

页脚

网易公司版权所有 ©1997-2017