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高通量的拟南芥耐旱表型筛选方法:Screening Arabidopsis genotypes for drought stress resistance.  

2010-10-29 14:01:45|  分类: 植物耐铝机理 |  标签: |举报 |字号 订阅

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       很不错的方法,值得借鉴!
Virginia Bioinformatics Institute, Virginia Tech, Blacksburg, VA, USA.

Type:  Journal Article

Abstract Highlight Terms These include Gene Ontology terms, Diseases, Genes/Proteins, Species and Chemicals.')" onmouseout="UnTip()" border="0"<
Species(2) Chemicals(1)
A high throughput drought screen is described for Arabidopsis that is based on a gravimetric method to monitor and control water content of the soil. To screen for plant growth under mild drought conditions, 30% of field capacity can be used, which is equal to 2 g H(2)O/g dry soil. The screen allows the testing of a large number of plants of different sizes to the same level of soil water. Therefore, response to drought of different genotypes can be compared. This method can be used for knockout or overexpression genotypes, which are evaluated for their drought response in terms of their growth measured by change in biomass.
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